FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_20.35100000038b4c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_20.35100000038b4c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences322908
Sequences flagged as poor quality0
Sequence length36
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGCCC300.001225050524.9985813
GTCCGGC300.001225050524.9985811
ACGGCCT402.463129E-422.4987228
GGCTAGA402.463129E-422.4987221
TCCGGCC350.003002516721.42735512
TAGGTAG504.591492E-520.9988085
AGACGGC504.591492E-520.9988086
AGTCCGG455.456965E-419.99886510
ACAGGCA455.456965E-419.9988658
GTAGACG608.225155E-619.9988654
TAGTCAG608.225155E-619.9988659
AGGCGTC701.4376492E-619.28760520
GATACGA701.4397847E-619.2846210
CACGCAG559.556371E-519.08982716
TATAAGG559.556371E-519.0898272
ACACGCA400.006502728418.74893415
GTCCTTA400.006502728418.7489341
TACAGGC400.006502728418.7489347
GACTGCA400.006502728418.7489347
CAGACCC400.006502728418.7489349
Produced by FastQC (version 0.11.2)