FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_19.35100000038b08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_19.35100000038b08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1600608
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG2850.017.8935815
GTATTAG4150.016.6254331
CTAGGCA1900.014.99903210
TATTAGG5100.013.8226382
GGTAGTC3150.013.8086337
GTATAGA4900.013.7746211
TAGTCAG3050.013.7696039
TTAGGTA3500.013.7134014
CGATCAC2250.013.33455724
ACCTAGC2151.8189894E-1213.25744329
ATCACAC3350.012.986670529
GACTAGG2203.6379788E-1212.953718
ACTATGC5050.012.7714538
CAGCGTG2950.012.7110439
TGTGCGG954.1912953E-412.63076410
TACTATG4900.012.550217
ATTAGGT4200.012.4991923
ACAGCGT3400.012.3521438
TCACACA3550.012.25502730
CTAGACT1354.213718E-612.2214344
Produced by FastQC (version 0.11.2)