FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_18.35100000038ac3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_18.35100000038ac3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1901890
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGGC851.6346858E-414.11728816
CACCCCC650.006390264713.8454387
TAGGTAG2800.013.3921655
GCCTCAC1352.8979775E-713.33404620
GACGGCT1056.757553E-512.8564787
GTATTAG5150.012.2323771
GGTAGTC2850.012.1046377
CTAATAC6950.011.8698893
TAGGGGT1406.323635E-611.7851064
TATTAGG5100.011.7640972
CTGAGCG1951.5341357E-811.5378669
ACCTAAT5600.011.5172621
TAGGCTG1703.768546E-711.4699955
ACTAGAC1602.2646964E-611.2494183
GTCTATA2950.011.1858631
ATACCGT4650.010.644616
CCTAGCG1704.674541E-610.58935830
AGACTCC1851.1219745E-610.5399956
CCTAATA6600.010.4540052
GTCTAGA2301.551598E-810.4342431
Produced by FastQC (version 0.11.2)