FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_17.35100000038a80.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_17.35100000038a80.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1431383
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCAG2300.018.9117229
GGTAGTC2700.016.6655037
TAGGTAG2650.016.4139485
GCTGCGC550.002102964316.3624928
TGTGCGA1004.2747466E-513.49905710
CAGCGCA800.00165762513.1240839
GTATTAG5400.013.0546441
TTAGGTA4300.012.9060754
GCCGCGT1404.4974877E-712.85983925
GTCTTAG1056.756431E-512.8562451
GACCCTG1201.695156E-512.4991267
GCCGTCT850.002612118112.35337322
TATTAGG5350.012.3355872
ATTAGGT5050.011.8803583
TACGGTG3050.011.8024547
TAGAGCG900.00400999811.6658525
CTATGCA3800.011.4465699
CTAATAC4200.011.4277733
AGTCAGA3950.011.39160910
ACTATGC4750.011.3676278
Produced by FastQC (version 0.11.2)