FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_16.35100000038a4d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_16.35100000038a4d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1593726
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGCG1451.8189894E-1015.51875130
TGTCCGA851.634462E-414.117249510
CTGTCCG750.001018466713.9996049
CACCTAG1752.0918378E-1013.7151928
GTATTAG3900.013.4615811
ACCTAGC1803.274181E-1013.33421129
TAGGTAG2151.8189894E-1213.255445
GGTAGTC2051.9463187E-1012.4386747
CGATCAC1857.021299E-912.16258124
TAGTCAG2052.562956E-911.7069879
TCGGGGC900.004008153511.66670322
ACTGCCC900.00400909311.6663378
ACTATGC4800.011.2496838
TGACGCG950.0059995911.052327
AGACTAG2351.875378E-910.8507587
CTAATAC5950.010.840033
CTATGCA3600.010.8330279
CAGTGCC1408.180077E-510.7139849
GACTAGG2104.5687557E-810.7139838
CTACACC2957.2759576E-1210.6776654
Produced by FastQC (version 0.11.2)