FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_15.35100000038a16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_15.35100000038a16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1617424
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTGC859.215108E-615.8810438
TAGGTAG2750.015.2714695
GGTAGTC2900.014.48156457
GACCGTG902.6548436E-413.3322337
GTATTAG4750.013.2620651
TAGTCAG2850.013.156819
ACCTAGC1151.5767383E-411.74106729
ACTGTGC1806.273149E-811.6657058
GTACGCC900.00401076111.6657059
CCCTAAG1703.7687096E-711.4696431
TATACTG7100.011.1962615
CCTAGCG1355.641042E-511.11294430
TCTAGAC1901.3264616E-711.0517213
GACCGTC2053.2081516E-810.9747057
AGGTAGT4000.010.8741046
TAGGACC1807.8858284E-710.8324394
CAGTGCA2251.1017619E-810.6657879
TTAGGTA4550.010.5485814
AGGACCG1000.00878504110.4991345
AGTCAGA3750.010.39914210
Produced by FastQC (version 0.11.2)