FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_14.351000000389c4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_14.351000000389c4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1479967
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCCGA650.00638776413.84596710
ACGGTGT2153.274181E-1112.557978
CTAATAC4050.012.2220573
CTGATCG1151.5791852E-411.7389729
CTATGCA2950.011.6947579
CCGCTCC900.0040084211.6665099
TCTTAGG2206.2755134E-1011.5907532
AGCGCAC1059.7147754E-411.42841710
CTTACAC2002.2268068E-811.2498483
TGCGACG1755.4719203E-711.1434630
GTATTAG4400.010.9089431
CTTATAC3900.010.7690853
TACAGGC2802.7284841E-1110.7141417
TATTAGG4650.010.6450182
TATACAG7500.010.5998565
CATATAC5400.010.5554133
GTCCTAC1000.00877996110.4998591
CGCTCCA1000.00877996110.49985910
ACCGTCC1602.7757571E-510.312368
TTAGGCT1653.894714E-59.9998654
Produced by FastQC (version 0.11.2)