FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_13.35100000038981.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_13.35100000038981.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1769934
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCC452.0427437E-523.331413
GCGCGGC601.8675785E-417.4985512
TGCGCGG653.440098E-416.15250811
ATGCGCG809.763782E-514.99875710
GTATTAG5250.013.4274581
TCTAGAC2350.012.76493
ACAGCGC1354.2152915E-612.221218
CTGTGCG1354.2152915E-612.221219
CGTGCAA1652.5598456E-711.817203510
CTAAGCC1406.3251373E-611.7847383
TCGTGCA1151.5805407E-411.7381589
TAGGTAG3650.011.5058975
GGTAGTC3350.011.1931017
GTCCGGC2301.3151293E-911.08603811
TACACTG3750.010.7991055
TAGACAG3500.010.7133985
TAGACTG2402.6575435E-910.624125
TAGTCAG2701.546141E-1010.5546819
CGCGGTA1000.00877642510.50061323
CCACGCC1000.00877642510.50061323
Produced by FastQC (version 0.11.2)