FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_96.35200000039e29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_96.35200000039e29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences694153
Sequences flagged as poor quality0
Sequence length36
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGT43090.6207565190959342No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTC32960.4748232738315616No Hit
GCATCCACCGTATGCGCACAAGCAGTGGGAGCCCAC30200.4350625870665401No Hit
CCGCTAACTTTTCAAACCGATGCAAACTCCGAATAC25700.37023538038443976No Hit
GTACCCGTGCTGCCGCTCGACTTGCATGTGTTAGGC25420.36620168752422017No Hit
GTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCG23540.33911832117703156No Hit
ACCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGG21840.314628043097127No Hit
GCATCCACCGTATGCGTACAAGCAGTGGGAGCCCAC21160.30483193186516516No Hit
GTCTAAGCCTTCCCACATCGTTTCCCACTTAACCAT19820.2855278303198286No Hit
GAGCCATGATCAGTCACACTGGAACTGAGACACGGT19590.2822144397560768No Hit
ATTAAGACTCGCTTTCGCTACGCCTCCCCTATTCGG18800.27083366347188587No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18090.2606053708620434No Hit
GGTCTATTCCCAGCGACTAGACGCCCTATTAAGACT17730.25541919432747534No Hit
CCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAG15600.22473431649794787No Hit
ATACAAAAGGTACGCAGTCACCCAACAAAGTGGGCT14340.20658269862695974No Hit
GTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATT14030.20211682438885953No Hit
AGCAGTGGGAGCCCACTTTGTTGGGTGACTGCGTAC13970.20125246163309818No Hit
GGCTTAGACAGCTAGGAGGTTGGCTTAGAAGCAGCC13900.2002440384180433No Hit
CTTTAAAGGGTGGCTGCTTCTAAGCCAACCTCCTAG13790.19865937336581416No Hit
CTATCTAGGTAGCGCCTCATGTATCACTGTAGGGGG13320.19188853177901702No Hit
GGAAACCTTGGTCTTTCGGCGTGGGTGTTTTTCACA13260.1910241690232557No Hit
GTACGAAGGTGCGCTCAGACCGGTCGGAAATCGGTC12690.18281272284352296No Hit
CTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT12500.18007557411694539No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGT12430.17906715090189051No Hit
GGATGTGATGAGCCGACATCGAGGTGCCAAACACCG12430.17906715090189051No Hit
GTATGGAAGGGCCATCGCTCAACGGATAAAAGGTAC12050.17359285344873535No Hit
CAGCGCCGCCATCGTTACGCCATTCGTGCAGGTACG11390.16408486313536064No Hit
ACCTACAAGTGCCGAGCATGGGAGACACACGGCGGG11310.16293237946101222No Hit
CGCTAACTTTTCAAACCGATGCAAACTCCGAATACC11140.16048335165302174No Hit
GTATAAAGGCAAAAGCGCGCTTGACTGCGAGACAGA11030.15889868660079262No Hit
ATTCCCAGCGACTAGACGCCCTATTAAGACTCGCTT10860.15644965879280218No Hit
CCTTCGTACTCCTCCGTTACTCTTTAGGAGGAGACC10730.15457687282198593No Hit
GGTTTAAGGTGGTAGGCTGAGATCTTAGGTAAATCC10580.15241596593258258No Hit
ATACAGAACCACCGGATCACTAAGACCTACTTTCGT9810.14132331056697875No Hit
CCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGGT9760.14060300827051098No Hit
CCCCTATTCGGTTAAGCTCGCCACTGAAAATAAGTC9730.1401708268926303No Hit
CCTCCTAGCTGTCTAAGCCTTCCCACATCGTTTCCC9650.13901834321828185No Hit
ATCGAGGACAGTGTATGGTGGGTAGTTTGACTGGGG9560.13772179908463986No Hit
TCCTATATCTACGCATTTCACCGCTACACAGGAAAT9260.13339998530583314No Hit
GCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCC9200.1325356225500718No Hit
GAACTCGGTCGTAGAGTATAAAGGCAAAAGCGCGCT9100.13109501795713624No Hit
GGTTCGCCTCTTGCACCTATGTATTCAGTACAAGAT8910.1283578692305587No Hit
GTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTA8660.12475635774821976No Hit
CCTTAAACCTGGACAACCAACGCCAGGCTGGCCTAG8470.12201920902164219No Hit
CTATTAAGACTCGCTTTCGCTACGCCTCCCCTATTC8400.1210107858065873No Hit
GTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTT8370.12057860442870663No Hit
GTTCTGTATGGAAGGGCCATCGCTCAACGGATAAAA8240.1187058184578904No Hit
GCTAGGAGGTTGGCTTAGAAGCAGCCACCCTTTAAA8050.11596866973131283No Hit
CCTTTATACTCTACGACCGATTTCCGACCGGTCTGA8030.11568054881272573No Hit
GTATAATGGGTCAGCGACTTATTTTCAGTGGCGAGC8030.11568054881272573No Hit
TGCGCACAAGCAGTGGGAGCCCACTTTGTTGGGTGA8020.11553648835343218No Hit
GATATAGGAAGGAACACCAGTGGCGAAGGCGACCAC7930.11423994421979017No Hit
GGCGTAAAGCGCGCGTAGGTGGTTTGTTAAGTTGGA7920.11409588376049662No Hit
CCGTTATCCGGATCGAGGACAGTGTATGGTGGGTAG7840.11294340008614816No Hit
CCTTTTGTATAATGGGTCAGCGACTTATTTTCAGTG7790.11222309778968038No Hit
CGCACAAGCAGTGGGAGCCCACTTTGTTGGGTGACT7700.11092655365603837No Hit
CTTTGGGACCTTAGCTGACGGTCTGGGTTGTTTCCC7500.10804534447016725No Hit
ATTTCCGACCGGTCTGAGCGCACCTTCGTACTCCTC7450.10732504217369945No Hit
GTCGTAGAGTATAAAGGCAAAAGCGCGCTTGACTGC7300.1051641352842961No Hit
CTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTAC7290.10502007482500256No Hit
CTTCCATACAGAACCACCGGATCACTAAGACCTACT7190.103579470232067No Hit
GAATTACTGGGCGTAAAGCGCGCGTAGGTGGTTTGT7190.103579470232067No Hit
GGCTTACAGAACGCTCCTCTACCGCATCATCAAAAG7040.10141856334266366No Hit
ATCCGGATCGAGGACAGTGTATGGTGGGTAGTTTGA7020.10113044242407654No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGTG3200.025.310133
GTACCCG3300.025.010271
CTAGATA300.001225189925.00306130
CCCGTGC3250.024.9189474
CCGTGCT3350.024.1750985
CCTAGAT402.4640997E-422.50275429
ACCTAGA402.4640997E-422.50275428
TACCTAG402.4640997E-422.50275427
ACCGTAT7300.021.9826587
CTCTTGC2050.021.9475848
CCGTATG7250.021.9274028
TCTTGCA2000.021.7463979
AGTCACA3050.021.63575712
TATGCGC4300.021.62432311
ACTCGGT1600.021.560483
GCACCTA2300.021.51972413
ATAGGGA350.003005344221.4281082
GCCTCTT2100.021.425026
CGTATGC7450.021.3387459
CCACCGT7450.021.3387455
Produced by FastQC (version 0.11.2)