FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_95.35200000039de4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_95.35200000039de4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences952870
Sequences flagged as poor quality0
Sequence length36
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGT74120.7778605685980249No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTC52090.5466642878881693No Hit
GTACCCGTGCTGCCGCTCGACTTGCATGTGTTAGGC40230.4221982012236717No Hit
GCATCCACCGTATGCGCACAAGCAGTGGGAGCCCAC38540.4044623086045316No Hit
GTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCG33430.35083484630642164No Hit
ATTAAGACTCGCTTTCGCTACGCCTCCCCTATTCGG30230.3172520910512452No Hit
GTCTAAGCCTTCCCACATCGTTTCCCACTTAACCAT27990.2937441623726217No Hit
GCATCCACCGTATGCGTACAAGCAGTGGGAGCCCAC26810.28136052137227535No Hit
ACCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGG26550.2786319225077923No Hit
CCGCTAACTTTTCAAACCGATGCAAACTCCGAATAC26230.27527364698227463No Hit
CCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAG26040.2732796708889985No Hit
GGTCTATTCCCAGCGACTAGACGCCCTATTAAGACT25330.2658284970667562No Hit
GAGCCATGATCAGTCACACTGGAACTGAGACACGGT24220.2541794788376169No Hit
CTTTAAAGGGTGGCTGCTTCTAAGCCAACCTCCTAG23730.24903711943916798No Hit
CTATCTAGGTAGCGCCTCATGTATCACTGTAGGGGG21420.22479456798933745No Hit
ATACAAAAGGTACGCAGTCACCCAACAAAGTGGGCT21170.22217091523502683No Hit
GTATAAAGGCAAAAGCGCGCTTGACTGCGAGACAGA21060.22101650802313014No Hit
GGCTTAGACAGCTAGGAGGTTGGCTTAGAAGCAGCC20760.21786812471795733No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20750.21776317860778488No Hit
GGATGTGATGAGCCGACATCGAGGTGCCAAACACCG20130.21125651977709445No Hit
GTATGGAAGGGCCATCGCTCAACGGATAAAAGGTAC19800.2077932981414044No Hit
CAGCGCCGCCATCGTTACGCCATTCGTGCAGGTACG19700.20674383703968013No Hit
CTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT19530.2049597531667489No Hit
AGCAGTGGGAGCCCACTTTGTTGGGTGACTGCGTAC19440.204015238175197No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGT19350.2030707231836452No Hit
GTACGAAGGTGCGCTCAGACCGGTCGGAAATCGGTC18880.19813825600554116No Hit
GAACTCGGTCGTAGAGTATAAAGGCAAAAGCGCGCT17990.1887980522001952No Hit
GGTTCGCCTCTTGCACCTATGTATTCAGTACAAGAT17740.18617439944588454No Hit
ACCTACAAGTGCCGAGCATGGGAGACACACGGCGGG17540.184075477242436No Hit
GGTTTAAGGTGGTAGGCTGAGATCTTAGGTAAATCC16990.17830344118295255No Hit
CCTTAAACCTGGACAACCAACGCCAGGCTGGCCTAG16510.1732660278946761No Hit
TCCTATATCTACGCATTTCACCGCTACACAGGAAAT15840.1662346385131235No Hit
ATACAGAACCACCGGATCACTAAGACCTACTTTCGT15420.1618269018858816No Hit
GTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTT15170.15920324913157094No Hit
GTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATT14830.15563508138570845No Hit
ATCGAGGACAGTGTATGGTGGGTAGTTTGACTGGGG14770.1550054047246739No Hit
GGAAACCTTGGTCTTTCGGCGTGGGTGTTTTTCACA14620.15343121307208749No Hit
CCTTCGTACTCCTCCGTTACTCTTTAGGAGGAGACC14490.15206691363984595No Hit
GTTCTGTATGGAAGGGCCATCGCTCAACGGATAAAA13710.1438811170463967No Hit
CGCTAACTTTTCAAACCGATGCAAACTCCGAATACC13510.14178219484294816No Hit
CTATTAAGACTCGCTTTCGCTACGCCTCCCCTATTC13390.14052284152087902No Hit
GCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCC13240.1389486498682926No Hit
ATTCCCAGCGACTAGACGCCCTATTAAGACTCGCTT12730.1335963982494989No Hit
GGCTTACAGAACGCTCCTCTACCGCATCATCAAAAG12620.13244199103760218No Hit
CCTCCTAGCTGTCTAAGCCTTCCCACATCGTTTCCC12590.13212715270708492No Hit
CCCCTATTCGGTTAAGCTCGCCACTGAAAATAAGTC12500.13118263771553307No Hit
GTCGTAGAGTATAAAGGCAAAAGCGCGCTTGACTGC12310.12918866162225695No Hit
GCTAGGAGGTTGGCTTAGAAGCAGCCACCCTTTAAA12060.1265650088679463No Hit
CCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGA11750.1233116794526011No Hit
CCTTTATACTCTACGACCGATTTCCGACCGGTCTGA11730.12310178723225623No Hit
CTTCCATACAGAACCACCGGATCACTAAGACCTACT11690.12268200279156652No Hit
CCTTTTGTATAATGGGTCAGCGACTTATTTTCAGTG11550.12121275724915256No Hit
GTATAATGGGTCAGCGACTTATTTTCAGTGGCGAGC11300.1185891044948419No Hit
TTCCTGTACTTCTGGTTATTGCGATGGAGGGACGGA11260.11816932005415219No Hit
GATATAGGAAGGAACACCAGTGGCGAAGGCGACCAC11140.11690996673208308No Hit
GGCTAGGCCAGCCTGGCGTTGGTTGTCCAGGTTTAA11020.11565061341001395No Hit
CCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGGT10960.1150209367489794No Hit
CGCACAAGCAGTGGGAGCCCACTTTGTTGGGTGACT10860.11397147564725513No Hit
TATCTAGGTAGCGCCTCATGTATCACTGTAGGGGGT10730.1126071762150136No Hit
ATTTCCGACCGGTCTGAGCGCACCTTCGTACTCCTC10430.1094587929098408No Hit
CTTTGGGACCTTAGCTGACGGTCTGGGTTGTTTCCC10430.1094587929098408No Hit
CTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTG10350.10861922402846137No Hit
CTTTAGGAGGAGACCGCCCCAGTCAAACTACCCACC10330.10840933180811653No Hit
TCTTTTGATGATGCGGTAGAGGAGCGTTCTGTAAGC10250.10756976292673713No Hit
GATAAAAGGTACTCCGGGGATAACAGGCTGATACCG10170.1067301940453577No Hit
GGCCTAGCCTTCTCCGTCCCTCCATCGCAATAACCA10110.10610051738432315No Hit
CCTAAAGAGTAACGGAGGAGTACGAAGGTGCGCTCA10010.10505105628259889No Hit
TGCGCACAAGCAGTGGGAGCCCACTTTGTTGGGTGA9980.1047362179520816No Hit
CCCTATTAAGACTCGCTTTCGCTACGCCTCCCCTAT9900.1038966490707022No Hit
GTGATACATGAGGCGCTACCTAAATAGCTTTCGAGG9840.10326697240966765No Hit
ATATTAACCTGTTTTCCATCGACTACGCTTTTCAGC9700.10179772686725366No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCCG4500.026.6768861
CCGTGCT5000.024.5978055
ACCCGTG4950.024.5432643
CCCGTGC5150.024.4638374
CCTAGAT501.6961312E-624.00163729
TACCTAG501.6961312E-624.00163727
ACTCGGT2450.023.875423
CTCGGTC2500.023.3979114
TATGCGC5700.022.1032911
ACCTAGA553.8816306E-621.8196728
CCTCTTG3300.021.8162367
TGTTAGG5450.021.74460429
CCACCGT10700.021.7270345
GTGTTAG5450.021.46935728
GTTAGGC5600.021.43003530
GTGCGGA350.003007336821.426669
TGTGTTA5350.021.30986627
ACCGTAT10950.021.2309827
CCGTATG10900.021.1907718
ACTCAGT504.6010144E-521.0003314
Produced by FastQC (version 0.11.2)