FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_94.35200000039da1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_94.35200000039da1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences679048
Sequences flagged as poor quality0
Sequence length36
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGT52930.7794736160035814No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTC25920.3817108658003558No Hit
GCATCCACCGTATGCGCACAAGCAGTGGGAGCCCAC22930.3376786324383549No Hit
CTTTAAAGGGTGGCTGCTTCTAAGCCAACCTCCTAG20410.3005678538188758No Hit
CCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAG18770.2764163947173101No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15920.23444587127861358No Hit
GTCTAAGCCTTCCCACATCGTTTCCCACTTAACCAT15810.2328259563388744No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGT15140.22295920170591768No Hit
ATTAAGACTCGCTTTCGCTACGCCTCCCCTATTCGG14980.22060296179356984No Hit
GTATGGAAGGGCCATCGCTCAACGGATAAAAGGTAC14910.2195721068319176No Hit
GTATAAAGGCAAAAGCGCGCTTGACTGCGAGACAGA14190.2089690272263522No Hit
CTATCTAGGTAGCGCCTCATGTATCACTGTAGGGGG14190.2089690272263522No Hit
GTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCG14130.20808543725922174No Hit
CTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT14110.20779090727017827No Hit
ATACAAAAGGTACGCAGTCACCCAACAAAGTGGGCT13670.20131124751122162No Hit
GGCTTAGACAGCTAGGAGGTTGGCTTAGAAGCAGCC13590.20013312755504764No Hit
ACCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGG13400.19733509265913454No Hit
ACCTACAAGTGCCGAGCATGGGAGACACACGGCGGG12070.17774884838774285No Hit
TCCTATATCTACGCATTTCACCGCTACACAGGAAAT11380.16758756376574263No Hit
GTACCCGTGCTGCCGCTCGACTTGCATGTGTTAGGC11160.1643477338862643No Hit
CCGCTAACTTTTCAAACCGATGCAAACTCCGAATAC11090.1633168789246121No Hit
CCTTAAACCTGGACAACCAACGCCAGGCTGGCCTAG11010.16213875896843816No Hit
GGTCTATTCCCAGCGACTAGACGCCCTATTAAGACT10950.1612551690013077No Hit
GTACGAAGGTGCGCTCAGACCGGTCGGAAATCGGTC10930.16096063901226423No Hit
AGCAGTGGGAGCCCACTTTGTTGGGTGACTGCGTAC10790.15889892908895983No Hit
GCATCCACCGTATGCGTACAAGCAGTGGGAGCCCAC10540.15521730422591629No Hit
CAGCGCCGCCATCGTTACGCCATTCGTGCAGGTACG10300.15168294435739446No Hit
GAGCCATGATCAGTCACACTGGAACTGAGACACGGT9750.14358336965869864No Hit
ATACAGAACCACCGGATCACTAAGACCTACTTTCGT9530.14034353977922032No Hit
GTTCTGTATGGAAGGGCCATCGCTCAACGGATAAAA9440.13901815482852464No Hit
GTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTT9250.13622011993261154No Hit
GATATAGGAAGGAACACCAGTGGCGAAGGCGACCAC9230.13592558994356807No Hit
CGTTAGCACCCGCCGTGTGTCTCCCATGCTCGGCAC9070.13356935003122017No Hit
GATAAAAGGTACTCCGGGGATAACAGGCTGATACCG9060.13342208503669845No Hit
GGCTTACAGAACGCTCCTCTACCGCATCATCAAAAG8780.12929866519008967No Hit
GAACTCGGTCGTAGAGTATAAAGGCAAAAGCGCGCT8740.12870960521200267No Hit
GCTAGGAGGTTGGCTTAGAAGCAGCCACCCTTTAAA8690.12797328023939397No Hit
GGTTTAAGGTGGTAGGCTGAGATCTTAGGTAAATCC8370.12326080041469822No Hit
CCTTTATACTCTACGACCGATTTCCGACCGGTCTGA8340.122819005431133No Hit
CCCTTTAAAGAAAGCGTAATAGCTCACTAGTCGAGT7900.11633934567217635No Hit
CCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGA7850.11560302069956764No Hit
GGATCACAGTCTGTTTGCCGACTCCCCGAAGCTTTT7790.11471943073243718No Hit
ATATTAACCTGTTTTCCATCGACTACGCTTTTCAGC7780.11457216573791543No Hit
CTTTAGGAGGAGACCGCCCCAGTCAAACTACCCACC7680.11309951579269802No Hit
ATGTATCACTGTAGGGGGTAGAGCACTGTTTCGGCT7670.11295225079817628No Hit
TATCTAGGTAGCGCCTCATGTATCACTGTAGGGGGT7630.11236319082008932No Hit
CTTCCATACAGAACCACCGGATCACTAAGACCTACT7490.11030148089678492No Hit
GCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCC7400.10897609594608924No Hit
ATCGAGGACAGTGTATGGTGGGTAGTTTGACTGGGG7400.10897609594608924No Hit
CTTCTAAGCCAACCTCCTAGCTGTCTAAGCCTTCCC7250.1067671210282631No Hit
CTTTGGGACCTTAGCTGACGGTCTGGGTTGTTTCCC7110.1047054111049587No Hit
CCTTTTGTATAATGGGTCAGCGACTTATTTTCAGTG7090.10441088111591522No Hit
CCCCTATTCGGTTAAGCTCGCCACTGAAAATAAGTC6950.10234917119261085No Hit
CCTTCGTACTCCTCCGTTACTCTTTAGGAGGAGACC6950.10234917119261085No Hit
CTATTAAGACTCGCTTTCGCTACGCCTCCCCTATTC6900.1016128462200021No Hit
ATTTCCGACCGGTCTGAGCGCACCTTCGTACTCCTC6840.10072925625287167No Hit
GTCGTTACTCATGTCAGCATTCGCACTTCTGATACC6830.10058199125834992No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCTA200.00514844929.9968345
GTACCCG1400.025.7153591
ACCCGTG1350.025.5528583
TGTAAGT300.001226792124.9973622
ACTCGGT1650.024.5428643
CCCGTGC1600.022.4976254
CTCGGTC1850.021.8895824
TATGCGC3750.021.5977211
TGTTAGG1550.021.29434829
GGTCCGA504.5975907E-521.0008751
AGCGTAT504.6028123E-520.99778410
GCCATAC8700.020.862941
GTGTTAG1600.020.628928
CCATACC8850.020.506312
TACCCTT8450.020.4120465
CCGTATG5150.020.3861968
CATGTGT1700.020.29795525
TGTGTTA1700.020.29795527
CTTGCAT1700.020.29496421
CATACCC8900.020.2225843
Produced by FastQC (version 0.11.2)