FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_93.35200000039d6d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_93.35200000039d6d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75718
Sequences flagged as poor quality0
Sequence length36
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6020.7950553369080007No Hit
GCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGT3420.4516759555191632No Hit
GCATCCACCGTATGCGCACAAGCAGTGGGAGCCCAC3200.4226207770939539No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTC2840.3750759396708841No Hit
ATACAAAAGGTACGCAGTCACCCAACAAAGTGGGCT2350.31036213317837236No Hit
GCATCCACCGTATGCGTACAAGCAGTGGGAGCCCAC2010.26545867561213976No Hit
CTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT1840.24300694682902346No Hit
CTTTAAAGGGTGGCTGCTTCTAAGCCAACCTCCTAG1790.23640349718693043No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGT1690.2231965979027444No Hit
GTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCG1500.19810348926279087No Hit
GGTCTATTCCCAGCGACTAGACGCCCTATTAAGACT1470.19414141947753505No Hit
CCGCTAACTTTTCAAACCGATGCAAACTCCGAATAC1380.1822552101217676No Hit
AGCAGTGGGAGCCCACTTTGTTGGGTGACTGCGTAC1240.16376555112390712No Hit
ACCTACAAGTGCCGAGCATGGGAGACACACGGCGGG1220.1611241712670699No Hit
GTATAAAGGCAAAAGCGCGCTTGACTGCGAGACAGA1220.1611241712670699No Hit
GTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTT1200.1584827914102327No Hit
GGCTTAGACAGCTAGGAGGTTGGCTTAGAAGCAGCC1180.15584141155339548No Hit
CCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAG1140.15055865183972109No Hit
CCTTAAACCTGGACAACCAACGCCAGGCTGGCCTAG1120.14791727198288387No Hit
GTATGGAAGGGCCATCGCTCAACGGATAAAAGGTAC1100.14527589212604666No Hit
CGCACAAGCAGTGGGAGCCCACTTTGTTGGGTGACT1080.14263451226920945No Hit
GTCTAAGCCTTCCCACATCGTTTCCCACTTAACCAT1050.1386724424839536No Hit
GTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATT1030.1360310626271164No Hit
ATTAAGACTCGCTTTCGCTACGCCTCCCCTATTCGG1010.13338968277027918No Hit
GTACGAAGGTGCGCTCAGACCGGTCGGAAATCGGTC1010.13338968277027918No Hit
CCTTTATACTCTACGACCGATTTCCGACCGGTCTGA1000.13206899284186058No Hit
ACCAGGAACCGTACCCCAAACCGACACAGGTGGTTG980.12942761298502337No Hit
CTTTGGGACCTTAGCTGACGGTCTGGGTTGTTTCCC970.12810692305660476No Hit
CGTTAGCACCCGCCGTGTGTCTCCCATGCTCGGCAC950.12546554319976755No Hit
CAGCGCCGCCATCGTTACGCCATTCGTGCAGGTACG900.11886209355767452No Hit
ATTTCCGACCGGTCTGAGCGCACCTTCGTACTCCTC900.11886209355767452No Hit
CGCTAACTTTTCAAACCGATGCAAACTCCGAATACC890.11754140362925593No Hit
CTTTAGGAGGAGACCGCCCCAGTCAAACTACCCACC870.11490002377241872No Hit
CCTTCGTACTCCTCCGTTACTCTTTAGGAGGAGACC860.1135793338440001No Hit
GTTTGGCACCTCGATGTCGGCTCATCACATCCTGGG840.11093795398716289No Hit
GGTACGCAGTCACCCAACAAAGTGGGCTCCCACTGC810.10697588420190708No Hit
GTTCTGTATGGAAGGGCCATCGCTCAACGGATAAAA770.10169312448823264No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCTT200.005101864730.01850730
AAATGCT200.00511839529.9986783
GTAAATG200.00511839529.9986781
TGGGGCG501.6636313E-623.99894322
ACCGTAT1100.023.1807967
CCGTATG1150.022.1729358
GTGGGAG1150.022.17293525
TGGGAGC1150.022.17293526
TCTCCTC553.7860264E-621.8316430
AGGGGAG350.002981346321.4276285
CGCCCCA350.002981346321.42762815
TATGCGT705.9231752E-821.42762811
ATAGGGG350.002981346321.4276283
AAGGCAT350.002981346321.4276284
GAATAGG350.002981346321.4276281
TAGGGGA350.002981346321.4276284
TCCTGTA350.002981346321.4276282
GCGCACA504.531674E-520.99907514
GTATGCG1250.020.39910110
CACCGTA1250.020.3991016
Produced by FastQC (version 0.11.2)