FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_92.35200000039d2a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_92.35200000039d2a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137684
Sequences flagged as poor quality0
Sequence length36
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGT7080.5142209697568345No Hit
GCATCCACCGTATGCGCACAAGCAGTGGGAGCCCAC4190.3043200371866012No Hit
CCGCTAACTTTTCAAACCGATGCAAACTCCGAATAC4130.29996223235815345No Hit
GTCTAAGCCTTCCCACATCGTTTCCCACTTAACCAT3570.2592893872926411No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTC3470.2520263792452282No Hit
CTTTAAAGGGTGGCTGCTTCTAAGCCAACCTCCTAG3170.23023735510298943No Hit
GTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCG2970.21571133900816364No Hit
GGTCTATTCCCAGCGACTAGACGCCCTATTAAGACT2940.21353243659393972No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2760.2004590221085965No Hit
CCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAG2440.17721739635687517No Hit
GGCTTAGACAGCTAGGAGGTTGGCTTAGAAGCAGCC2420.1757647947473926No Hit
ACCTACAAGTGCCGAGCATGGGAGACACACGGCGGG2290.16632288428575578No Hit
ACCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGG2200.15978617704308418No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGT2150.1561546730193777No Hit
AGCAGTGGGAGCCCACTTTGTTGGGTGACTGCGTAC2120.15397577060515383No Hit
ATTAAGACTCGCTTTCGCTACGCCTCCCCTATTCGG2110.15324946980041254No Hit
GTATGGAAGGGCCATCGCTCAACGGATAAAAGGTAC2070.15034426658144737No Hit
GTACGAAGGTGCGCTCAGACCGGTCGGAAATCGGTC2020.14671276255774093No Hit
GCATCCACCGTATGCGTACAAGCAGTGGGAGCCCAC1980.14380755933877576No Hit
CGCTAACTTTTCAAACCGATGCAAACTCCGAATACC1910.1387234537055867No Hit
GTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTT1890.13727085209610412No Hit
CTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT1870.13581825048662152No Hit
CCTTAAACCTGGACAACCAACGCCAGGCTGGCCTAG1870.13581825048662152No Hit
GGTTTAAGGTGGTAGGCTGAGATCTTAGGTAAATCC1800.1307341448534325No Hit
GTATAAAGGCAAAAGCGCGCTTGACTGCGAGACAGA1790.1300078440486912No Hit
CAGCGCCGCCATCGTTACGCCATTCGTGCAGGTACG1720.12492373841550217No Hit
GGCTTACAGAACGCTCCTCTACCGCATCATCAAAAG1680.12201853519653699No Hit
ATACAGAACCACCGGATCACTAAGACCTACTTTCGT1590.11548182795386537No Hit
CTATCTAGGTAGCGCCTCATGTATCACTGTAGGGGG1560.1133029255396415No Hit
TCTCTACCCAACCACCTGTGTCGGTTTGGGGTACGG1530.11112402312541762No Hit
GGACCGAACTGTCTCACGACGTTCTAAACCCAGCTC1510.10967142151593504No Hit
GTTTTCCATCGACTACGCTTTTCAGCCTCGCCTTAG1430.1038610150780047No Hit
ATACAAAAGGTACGCAGTCACCCAACAAAGTGGGCT1420.10313471427326343No Hit
TCTTTTGATGATGCGGTAGAGGAGCGTTCTGTAAGC1420.10313471427326343No Hit
GTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATT1390.10095581185903954No Hit
GCTAGGAGGTTGGCTTAGAAGCAGCCACCCTTTAAA1380.10022951105429825No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTGA200.005131716429.9999986
CTGCCTC200.005131716429.99999819
TGCCTCC200.005131716429.99999820
AGGAGAA402.4522367E-422.49999817
TTTCAAA755.151378E-922.010
CTATGTA350.002992883821.42857217
AACTTAC350.002992883821.4285722
CCCAAGG350.002992883821.42857220
CCTATGT350.002992883821.42857216
TATGTAT350.002992883821.42857218
AATCGGT504.5649504E-521.029
TATGCGC801.0841177E-820.62499811
CGCTAAC951.6916601E-1020.5263162
TACCCTT1101.8189894E-1220.4545465
GAGAACC455.4330635E-420.019
CATACCC1200.020.03
CCGCTAA1003.3651304E-1019.51
AACTTTT852.1764208E-819.4117646
ACTTTTC852.1764208E-819.4117647
CCACTTA701.4267771E-619.28571525
Produced by FastQC (version 0.11.2)