FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_91.35200000039ce5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_91.35200000039ce5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121237
Sequences flagged as poor quality0
Sequence length36
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGT10790.8899923290744575No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTC4860.40086772190007997No Hit
GCATCCACCGTATGCGCACAAGCAGTGGGAGCCCAC4750.39179458416160085No Hit
CCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAG3650.3010632067768091No Hit
GTCTAAGCCTTCCCACATCGTTTCCCACTTAACCAT3560.2936397304453261No Hit
CCGCTAACTTTTCAAACCGATGCAAACTCCGAATAC3470.28621625411384316No Hit
GGTCTATTCCCAGCGACTAGACGCCCTATTAAGACT3260.2688948093403829No Hit
GTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCG3230.26642031722988857No Hit
CTTTAAAGGGTGGCTGCTTCTAAGCCAACCTCCTAG3160.2606465023054018No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGT2920.24085056542144725No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2780.22930293557247378No Hit
GGCTTAGACAGCTAGGAGGTTGGCTTAGAAGCAGCC2630.21693047502000215No Hit
CAGCGCCGCCATCGTTACGCCATTCGTGCAGGTACG2620.21610564431650403No Hit
GCATCCACCGTATGCGTACAAGCAGTGGGAGCCCAC2390.1971345381360476No Hit
GTATGGAAGGGCCATCGCTCAACGGATAAAAGGTAC2290.1888862311010665No Hit
GTATAAAGGCAAAAGCGCGCTTGACTGCGAGACAGA2170.17898826265908924No Hit
ATTAAGACTCGCTTTCGCTACGCCTCCCCTATTCGG2150.177338601252093No Hit
ACCTACAAGTGCCGAGCATGGGAGACACACGGCGGG2100.17321444773460248No Hit
CTATCTAGGTAGCGCCTCATGTATCACTGTAGGGGG1970.1624916485891271No Hit
AGCAGTGGGAGCCCACTTTGTTGGGTGACTGCGTAC1880.1550681722576441No Hit
ACCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGG1840.1517688494436517No Hit
ATACAAAAGGTACGCAGTCACCCAACAAAGTGGGCT1830.1509440187401536No Hit
CTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT1830.1509440187401536No Hit
GTACGAAGGTGCGCTCAGACCGGTCGGAAATCGGTC1820.1501191880366555No Hit
CGCTAACTTTTCAAACCGATGCAAACTCCGAATACC1630.13444740467019145No Hit
GTTCTGTATGGAAGGGCCATCGCTCAACGGATAAAA1630.13444740467019145No Hit
GGTTTAAGGTGGTAGGCTGAGATCTTAGGTAAATCC1610.13279774326319524No Hit
CCTTCGTACTCCTCCGTTACTCTTTAGGAGGAGACC1600.13197291255969712No Hit
CCTTAAACCTGGACAACCAACGCCAGGCTGGCCTAG1580.1303232511527009No Hit
GCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCC1490.12289977482121794No Hit
GTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTT1470.12125011341422172No Hit
CTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTAC1470.12125011341422172No Hit
ACCATACACTGTCCTCGATCCGGATAACGGACCTGA1450.11960045200722551No Hit
GAGCCATGATCAGTCACACTGGAACTGAGACACGGT1420.11712595989673119No Hit
GCTAGGAGGTTGGCTTAGAAGCAGCCACCCTTTAAA1420.11712595989673119No Hit
ATACAGAACCACCGGATCACTAAGACCTACTTTCGT1400.11547629848973498No Hit
GTCGTAGAGTATAAAGGCAAAAGCGCGCTTGACTGC1340.11052731426874633No Hit
CCTCCTAGCTGTCTAAGCCTTCCCACATCGTTTCCC1330.10970248356524824No Hit
GGCTTACAGAACGCTCCTCTACCGCATCATCAAAAG1260.10392866864076149No Hit
GCCCCAGGATGTGATGAGCCGACATCGAGGTGCCAA1250.10310383793726338No Hit
CTTCCATACAGAACCACCGGATCACTAAGACCTACT1240.10227900723376528No Hit
TGCGCACAAGCAGTGGGAGCCCACTTTGTTGGGTGA1230.10145417653026716No Hit
CTTTGGGACCTTAGCTGACGGTCTGGGTTGTTTCCC1230.10145417653026716No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACTG200.00513001229.9991745
ATTCGTG659.804353E-1025.38391722
CGTTACG659.804353E-1025.38391713
CCGCTAA900.025.0199551
ATCGTTA601.1690645E-824.99931111
TTCGTGC702.3155735E-923.57078223
GTTACGC702.3155735E-923.57078214
TTACGCC702.3155735E-923.57078215
CTTCCCA652.73576E-823.0762889
CCATCGT652.73576E-823.0762889
GCCATAC2000.022.517961
GGTCTAT402.4366766E-422.517961
TCTATTC402.450344E-422.499383
CATTCGT755.138645E-921.99939721
CCATTCG755.138645E-921.99939720
CATACCC2050.021.9506173
AGGAGAA350.002991318721.42798217
CCCTAAG350.002991318721.42798213
CCAGCTA350.002991318721.42798224
CGTACAA350.002991318721.42798215
Produced by FastQC (version 0.11.2)