FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_89.35200000039c6e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_89.35200000039c6e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences717520
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20700.2884937005240272No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCGGG2000.022.50209225
CCGGCCC2100.021.42757613
AGCCCGG1700.021.17401110
CGGCCCG2000.020.99902514
CCCGGGG1900.020.52822326
CCCGGAG1900.020.52822317
GGGCCCG2350.019.78631423
GAGCCCG1750.019.7119969
GCCCGGA2000.019.50181216
CCCGGCC2200.019.09002112
AGGAGCC2050.019.022187
TCCTAGG400.006512534318.7478222
CCGGGGC2100.018.57315427
GGAGCCC1950.018.4593948
GCCCGGC2400.018.12289411
AGGGCCC2350.017.87275522
CGGAGGG2200.017.7289219
CCGGAGG2300.017.61033218
GAGGGCC2300.017.60910621
AAGGAGC2200.017.0434746
Produced by FastQC (version 0.11.2)