FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_88.35200000039c38.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_88.35200000039c38.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1035077
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25450.24587542762519116No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTAGG750.001015733314.0038111
CTGTGCG1056.755733E-512.8556739
CTGTGTA1252.5760706E-511.9986289
GGACTTA900.00401004711.6653346
GTCATAT2155.3751137E-911.165831
GACTTAC1253.424336E-410.7987657
GCGTTAA1000.00875839710.5028591
TGGACAG1150.00207642210.4335915
ATACTGT2957.2759576E-1110.1683296
GTATTAA3305.456968E-1210.0027231
CCCTTAG1501.6381123E-410.0027231
TATACTG2701.724402E-99.99885755
CTATACT2609.8789315E-99.8065714
CATATAC4500.09.6655623
GTCTATG1409.683446E-49.6454821
TATAATA5400.09.4433652
TATACAG3502.0008883E-119.4274935
AATACAG3502.0008883E-119.4274935
ACGGTGT2557.655581E-89.4106888
GTATTAT4150.09.4001481
Produced by FastQC (version 0.11.2)