FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_87.35200000039be6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_87.35200000039be6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1236955
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25790.2084958628244358No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGC653.4363853E-416.15415223
CGCGCAT800.001657809413.12365610
CTAGCAC1456.902228E-712.4130243
GCCGCGT850.002611293712.35367425
TGGCCGG850.002612877912.352674514
ACAGGGC900.00401044611.6654738
TAGACTG1303.8480626E-511.5372815
TAAAGGT3800.011.05154
CGCGTCG1000.00877385810.50062327
TATTATA6600.010.4538972
CTGGACT1150.002076709410.4337144
CCTATAC1304.917697E-410.3839723
CTATACT2905.2750693E-1110.3437684
TACCCTA1756.617931E-610.284665
CAGCGTG1200.00295663839.9989779
TAGGTAG2003.044608E-69.74900155
TATTAGG3254.5474735E-119.6917072
CTAATAC2957.748895E-109.66041853
TGGCGAC1250.0041386039.60056930
CGTCGCC1250.0041399859.60018121
Produced by FastQC (version 0.11.2)