FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_86.35200000039ba3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_86.35200000039ba3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1113470
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20690.18581551366449028No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTGC600.003757488414.99788
GACGCGC650.00639209113.8441238
CCAGCGC902.6551224E-413.3313788
GGTCTAA850.002607131612.3561231
ACTGATC1354.2143347E-612.2204298
CACACTG1501.0407239E-611.99823955
GTATAGA2553.6379788E-1211.76773551
CTACACA1303.848999E-511.5367694
ACTATGC2900.011.3776418
CTAAGAC1459.344762E-611.3776413
CTAATAC3200.011.248353
GTCTTAT2053.187779E-810.9784361
CTATGCA2607.8216544E-1110.959939
CTAACCA1100.001434376910.9074914
ACTGGTC1253.425801E-410.7984168
TAATACT4700.010.5303714
TGCGCTA1000.00878634110.4984610
ATACCGT2002.689685E-710.498466
TGTACTG2301.5506885E-810.4332515
TAGACAG1451.16806856E-410.343315
Produced by FastQC (version 0.11.2)