FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_85.35200000039b6f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_85.35200000039b6f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences460070
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12580.2734366509444215No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCAGG350.003004265721.42756311
TGATACC600.003750539214.9992933
TAAGAGT809.735848E-514.9992934
CTAAGAG650.00638036613.8455033
TTAGAGT800.001654367113.1243824
ACACCAT1006.4385706E-411.9994356
TATACTG1501.0350759E-611.9994355
GTGTTTA900.003986024811.6724611
GTACACA900.004002250711.6661174
GTATAAC1059.6512266E-411.4342461
CTATTAT1459.245216E-611.3849611
TTGTACT1253.4144102E-410.7994914
TATAATA3550.010.5628822
CCAGTAG1000.00876671610.4995063
TTATAAC1150.00207149610.4342913
TATATGG1200.002949279.9995292
ATTATCC1200.002949279.9995293
CTAATCA1200.002949279.9995294
GTATTAT1552.2680678E-49.6822251
AAGAGTG1409.690162E-49.6424045
Produced by FastQC (version 0.11.2)