FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_84.35200000039b2c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_84.35200000039b2c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences751240
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13010.17318034183483308No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCAG706.040318E-414.9977385
CCGCCGT750.001016993914.00068324
CTGGCGC650.00638126913.84682923
TAGTCAG1151.0934467E-513.0415119
TCACACA1056.723826E-512.86119530
GAGAGTC1056.7532514E-512.8552037
GGTAGTC1201.6940328E-512.4981157
TAGGTAG1252.5748272E-511.998195
GCCGTTT1151.5733589E-411.74204826
TCCCGCC900.004003414411.66723522
AGCCAGT900.00400939311.6649066
GTATAGA2103.7107384E-911.4314121
CCTTAAG1100.001428713610.9118031
TTACACC1551.9546702E-510.6435554
TAGAGTG1000.00878214810.4984155
ACCCTCT1000.00878214810.4984157
CTAGTTG1000.00878214810.4984153
GTTGTAG1150.002069028310.4373761
CTCTAGT1150.002069028310.4373761
TGTAGAC1304.916909E-410.3830493
Produced by FastQC (version 0.11.2)