FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_83.35200000039ae7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_83.35200000039ae7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences875579
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16910.19312934640963295No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGACCG601.8660957E-417.4987015
CTGGCCT550.00210214816.3624214
TTGTGCG706.038225E-414.9988869
GTGGCGT850.00261062212.35343530
GAGGTTA850.002612859712.3520249
CGATCCC850.002612859712.35202410
TAGGTAG1101.04307335E-412.2718165
CTATGCA1501.0389704E-611.9991099
ACTATGC2503.6379788E-1211.9991098
CTTACAC1501.0389704E-611.9991093
GTCTATA1755.439033E-711.1471231
GGCGTCC950.00599288711.05307421
TACGCTG950.005997983711.0518115
CTAATAC2651.09139364E-1010.7539193
GTCTTAT1551.9441843E-510.6492371
CCTATAC1150.00207518110.4340093
TATACCG1304.915179E-410.3838445
GTATTAA2752.1282176E-1010.3676031
ATCACAC1501.641299E-410.000429
TTCTATA3451.8189894E-129.9992582
Produced by FastQC (version 0.11.2)