FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_81.35200000039a60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_81.35200000039a60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences377683
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4580.12126571754619614No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCAG452.0394085E-523.3281869
GTATTAG953.807145E-918.9532241
GTATAGA901.5713686E-515.0046371
GTTATAG706.0094474E-415.0046371
CTAATAC901.5792653E-514.9966913
CAGATCC600.00375321514.9966899
TGGATAG750.001017419613.9969115
TCAGATG650.006384913313.84309912
TAAGGAG650.006384913313.8430995
GGTAGTC650.006384913313.8430997
TATTAGG1004.264494E-513.4970222
TAAGCAG1004.264494E-513.4970225
TTAGGTA902.6495126E-413.3303924
GTATTAA1251.7612838E-613.2040811
CTATGCA800.001655574313.1221039
ATTAGGT800.001655574313.1221033
ACTGAGC800.001655574313.1221038
CTTCTAA1056.706996E-512.8611171
TCTAATA1056.74038E-512.8543062
CTCTAAG954.183646E-412.6287912
Produced by FastQC (version 0.11.2)