FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_80.35200000039a2d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_80.35200000039a2d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences258737
Sequences flagged as poor quality0
Sequence length36
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6470.2500608726235521No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGAG350.003000269621.4281563
CCATAAG400.006483413318.756891
TCACACA400.006497906518.7496430
CTAAGGG400.006497906518.749643
TTATCGC400.006497906518.7496428
ATAAGAC400.006497906518.749643
GTGTTAA705.9977267E-415.005511
ACCTAAT600.003734316215.005511
GTATAGA600.003734316215.005511
GTATTAG750.001011391314.0051431
GTATATA750.001011391314.0051431
CCTTAAA650.006352949413.851241
CATAAGG650.006361094313.8485622
ACACTGT650.00636924813.8458866
CTAATAC800.001650615213.1247473
ATAAGGA1056.7117566E-512.85689453
ATTATAG954.168647E-412.6313353
GAACAGT850.00260321212.3527026
ATCTTGA1252.5459867E-512.0044091
AATACTA900.0039933311.6664415
Produced by FastQC (version 0.11.2)