FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_79.352000000399e8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_79.352000000399e8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences715745
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17360.24254448162404207No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA350.003006235721.42712422
GTCCTAA802.4750625E-718.7631531
ACCGGGA400.006505437718.75135426
CTAATAC2400.016.8738613
CTTAAGG755.619353E-515.9989193
GGGCTCA755.619353E-515.99891911
TAGGTAG755.619353E-515.9989195
ACTGCTC1052.4760084E-715.7132258
ATCACAC1900.015.00213229
GGTAGCC706.036007E-414.9989878
ATTATAC2500.014.3990273
TCACACA1802.1827873E-1114.16768930
AAGTGCG750.001017806113.9990547
GTAGACG650.00638580113.8452194
ATACTAT2750.013.6354426
CCTAATA2250.013.3324332
AAGCGAC800.001653573913.12686527
GTATATG1507.2976036E-813.0091191
CTATGCA2350.012.7650969
AATACTA2950.012.7110075
Produced by FastQC (version 0.11.2)