FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_78.352000000399a5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_78.352000000399a5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2360542
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39520.1674191774600918No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGTA1006.459422E-411.998679
TCGGGGG1151.5804454E-411.73857516
GCCGTGC1151.5810688E-411.7380778
CTAATAC6800.011.6901273
TAGACTG2503.8198777E-1111.3987375
GGCTCGG950.00600378311.05164053
CTGAGCG1501.3625862E-510.998789
CGGGGGC1253.425721E-410.7994917
GTATTAG6150.010.7368851
TAATACT10100.010.54338554
CAGGACT2209.055293E-810.226144
CCGTTCC1952.2064542E-69.9988929
TAGGTAG2854.1472958E-109.9988915
TATACAG9650.09.9470845
CCTATAC4100.09.8771633
CTATGCA5400.09.7211449
ACTATGC6850.09.6341728
GTATTAT12000.09.6296441
GTCTTAA3603.6379788E-129.5879581
CCGTGCA1450.00134069549.3093139
Produced by FastQC (version 0.11.2)