FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_77.35200000039961.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_77.35200000039961.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1637033
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23060.1408646007746942No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAGTC2400.014.3736257
ATACCCG952.6388627E-514.2091675
GTATTAG4000.012.7526741
ACCGTGT1406.32523E-611.7845868
GACCGTG1151.5805906E-411.7380077
CCGTGCT1151.5805906E-411.7380079
TAGGCGG900.004011193311.665555
GTGGTCG900.004011193311.665559
TAGTCAG2701.8189894E-1211.665559
TAGGTAG2651.09139364E-1010.7536885
GGTCTAT1851.1178472E-610.5427511
ACTATGC3850.010.5184758
GTGCGAC1304.911359E-410.38584129
GTACTAG3051.2732926E-1110.3300361
TGCGACG1356.9509423E-410.00118230
AGGCGTC1356.9567986E-410.00026520
GTACGCA1501.6455332E-49.999042511
CCTAATA5600.09.91006852
GTATAGA5000.09.9020771
GACATAG3203.274181E-119.84491330
Produced by FastQC (version 0.11.2)