FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_76.3520000003993b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_76.3520000003993b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences470213
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8740.18587321065134313No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCAC400.006504775518.75006718
ACACCGT550.002100601616.3619546
AATACAG1256.686605E-915.5983965
TAGAACT600.00375209414.9984584
TCAGGAC650.006382998513.844733
GAGAAGC954.1753743E-412.63296826
TATACTG1456.8752706E-712.4125175
GTTCTAT1151.5605701E-411.7504191
TAAACAG900.004004140411.6654685
TAATCTG900.004004140411.6654685
GTATATG2002.1789674E-811.2608181
AACCTGT1100.001431181810.9079696
TCATAGA1000.00877082110.4989212
GTATTAC1150.002054337910.4448171
GTATTAG1451.151798E-410.3547741
GTATTAT2053.6994788E-710.2537531
ATACAGG1653.883167E-59.9989736
ATAATAC2301.7179264E-79.7816033
TTAATAC1409.695953E-49.6418663
TAGATTG1250.00413606879.5990135
Produced by FastQC (version 0.11.2)