FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_75.352000000398f6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_75.352000000398f6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3148581
Sequences flagged as poor quality0
Sequence length36
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG47090.1495594364572485No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAGCG800.001658294613.1241889
ACCGCTC950.00600329811.0519488
GACCGTC1100.001434624210.9084157
CAGGGCA1451.1686161E-410.3441879
GTCCTAG1356.9337944E-410.0044651
ACCGTCC1501.6460674E-49.9993818
TAGACGG1200.00295781379.999385
GTAGGAC2905.693437E-109.8271343
GTATATA20300.09.7580491
GTATAGG5250.09.7186221
TATACTG9000.09.6660685
GTCTTAG1952.3775294E-59.234891
GTCTTAC4250.09.1805681
GTCCAGG1801.0054776E-49.1707581
ACAGCGG1654.287915E-49.0903468
TAGACTG3654.7293724E-119.0405365
TAGAACG2003.2234508E-58.9994434
GCTGACC1350.00780871328.8883388
TAATACT11650.08.883574
TTAGCCC2054.30907E-58.7799444
Produced by FastQC (version 0.11.2)