FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_74.352000000398b2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_74.352000000398b2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1473389
Sequences flagged as poor quality0
Sequence length36
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24480.16614756863258787No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCAGA1551.5351579E-611.6184241
CCTGTAC1202.3471781E-411.2488553
CCTAGGA1100.001434316410.9079812
GGGTTAG1253.407562E-410.8051341
CTTACAC1951.8985156E-710.7681343
AGTACAG2402.6557245E-910.6239195
CTAGGAC1000.0087856810.4989323
GTTCTAG1304.894591E-410.3895521
TAGACAG1653.8984614E-59.9989825
ACACAGT1501.6453804E-49.9989826
CTTATAC3152.3646862E-119.9989823
TAGGGGT1356.964126E-49.9989814
GTGTAAG2003.023697E-69.7546351
TATACAG5700.09.7358515
CAGTGGG1552.2894764E-49.6764349
GTACTAT2803.2032403E-99.6474411
CAGTGCA1409.7218406E-49.6418759
TAGACTG1250.00414490759.5990235
GACTGTA1250.00414490759.5990237
ATACCGC1250.00414490759.5990236
Produced by FastQC (version 0.11.2)