FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_73.3520000003987f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_73.3520000003987f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences719290
Sequences flagged as poor quality0
Sequence length36
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22660.3150328796452057No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATACGT750.001018222513.9983135
GGATAGT954.1882886E-412.6300576
CTTATAC954.1882886E-412.6300573
CTAGACC850.002612982612.3514534
TCTAATC850.002612982612.3514533
GAGTAGA1006.4164813E-412.0060651
TATATGA1151.5785951E-411.7377172
GCCCACG900.004008199611.665267
GTTCAGG1551.5309961E-611.6187731
GTACAGA1202.3308316E-411.2556871
TCTAGAC1202.344409E-411.2486453
TATAATA1202.344409E-411.2486452
TAGACTC1100.001432993310.9077765
TCCTTAT1253.402036E-410.8054591
CCAAGAC1253.4217894E-410.7986983
TGTAGGA1253.4217894E-410.7986982
AACCGCT1000.00877955910.4987357
CCGTGTG1000.00877955910.4987359
ATGACAG1000.00877955910.4987355
ACCCTCT1150.002075204410.4335256
Produced by FastQC (version 0.11.2)