FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_72.3520000003983c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_72.3520000003983c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72
Sequences flagged as poor quality0
Sequence length36
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTTCCTCCCCTCCTGTTGATGCGGGTAAGGGGTG11.3888888888888888No Hit
GTTCGCACGCGGACCAGAAGAACATAGGAGAAAAAA11.3888888888888888No Hit
GGGTTGTTGGGGAGCTGGGTGTTGGGAGCTGGGTCA11.3888888888888888No Hit
GTCCAACATCAACAACAAAGTGTGTCCTATGGTAGG11.3888888888888888No Hit
TTTCAAAAAGGATCAGTGAAAGTGTCGGTAAAGCTA11.3888888888888888No Hit
GGCCGTCTGCGTTCATATCGAAACTTCTTGGAATCG11.3888888888888888No Hit
GTTATTAAGAGTGTTAATCTCGGTGATTTCTTTATA11.3888888888888888No Hit
AAGGAGGCCAATCAGGAAGTATAGCCACATAGTAAA11.3888888888888888No Hit
AAAAGGACGCAGCAAACTTCTCTTATCTACGAGATT11.3888888888888888No Hit
GAGGACAGCGACACGTGCATTCATGGTAGTGCTAAT11.3888888888888888No Hit
CCTATAACACGAAGCCTCAATACAAAAAATAACCAC11.3888888888888888No Hit
ACATTGTAACATCCAATCATATCTAAAAAAAAACAC11.3888888888888888No Hit
ATATTATAAAGAATTGCCCACCGTCTCCAAGAGCCG11.3888888888888888No Hit
AACAAAGTATTTGTTTAAAATGGTAAAGTTTGTCCT11.3888888888888888No Hit
TGCTAATACTGAACGGAAATAATTGTAATGTGACAT11.3888888888888888No Hit
AATGCATCCTTCTGAGCGTGGGACAAAAAGCTGGTT11.3888888888888888No Hit
GCTGGAGAGGATTAGACGGTGTAATGAAGGGTGTGG11.3888888888888888No Hit
AGGCGGGGGGATAAGGAAGGGGTGTGGTAAGAGGGT11.3888888888888888No Hit
CCTATGCGTCGTTGCGGATAGGCGAAATCGGCGTCC11.3888888888888888No Hit
GAACAGTACTGAATGACATCATATATCGAAAGGCTC11.3888888888888888No Hit
AAATAGAATCTGTTCCACCGGTGAAACTTAGCTTTT11.3888888888888888No Hit
CTCATAGCCTAACTCCTTTGCGGATTCGTTTACTAG11.3888888888888888No Hit
GAAGTAGTTTAACTCACCGTAAAATCGATATAAATT11.3888888888888888No Hit
GTAAATAAAAATTCTCATCTATGGAAAGTAAAAGGT11.3888888888888888No Hit
GAACTGGTGGTTGAGGAAATCAAAGATCGAGGCCTG11.3888888888888888No Hit
AGGTGGTAGATAGGGAAAGAGGGTTTCACGTAAAAC11.3888888888888888No Hit
GGCTATTCTCTGGCGGATACTATCGCATGTTTTGTC11.3888888888888888No Hit
ATCATCATCATCGTGATTAAGAAAAACAAACGAATC11.3888888888888888No Hit
TAGAATACGTCTCATTAGGTGCAACTATGTGATAAA11.3888888888888888No Hit
TTCCTCACCACAGACAAGCAATTCAAGTTACTTTAC11.3888888888888888No Hit
AGTAAGGAGGAGGCCTTGGTTCAGGCGTTTTGATCT11.3888888888888888No Hit
CTCCTCCTACAAGTAATGGTAAGCGAGGCAGGAAGA11.3888888888888888No Hit
ATTCACATACAGTCTTCCAGTGATTAAAATGACTTC11.3888888888888888No Hit
GACTAAAGGATAGATAGGCAACACTTTCATGGTTAG11.3888888888888888No Hit
ACTCTGTGGTCTGGACAGGCTTGTATTCATGAGGCG11.3888888888888888No Hit
GCTATGAAAAACTGAAGAAGATTACTAAATCGATTG11.3888888888888888No Hit
TCATGCACTTTCTCTTTGTATTCATCTTCATCTATT11.3888888888888888No Hit
GTAATGCCCCCTCAGGACAAGTGTCTATATATACAT11.3888888888888888No Hit
CTCGAAGCAGAGTTAAGCCAAGTAGTTCCCATTCCA11.3888888888888888No Hit
ATAAATAACCAGAGCACTTTTCTTATCCAATGGCTC11.3888888888888888No Hit
ATCTTCATCTTTAGATGGGTCCATGTGAAAGCTCAA11.3888888888888888No Hit
ATAATAAAAATAAAATACTAAGAAATATTATAACTC11.3888888888888888No Hit
GAGTATAGGTAACACGCTTGAGCGCCATCCATTTTC11.3888888888888888No Hit
GTTTGGGAATGCAGCTCTAAGTGGGTGGTAAATTCC11.3888888888888888No Hit
GGGCGTGAGTGATATTGAAATATTTAAAGACACTGT11.3888888888888888No Hit
GGGTGACGGTCGGCTGCGCGGCTTCACTCACGGCAG11.3888888888888888No Hit
GGTCAAAGTCATTGGAGGCCATGTTTGGGGGTGGGG11.3888888888888888No Hit
ACGCTATGAACTAACTCAAAAAGCTTCTCAAGTTTC11.3888888888888888No Hit
GCACAAGTTCTTGTAGGGCAACCGTTTGATACAACA11.3888888888888888No Hit
ATACAAGAGGGTTCTGTGAATAATGGCTGCAAGTGA11.3888888888888888No Hit
GGCTGACACTGAAGAAGATATCAAGATCATAACGTA11.3888888888888888No Hit
GTACCACACATATACCCAAGATCTCAAGGCTGTTCA11.3888888888888888No Hit
GTATAAAAACATATCGGATGCTTCTTTATTGTGGGG11.3888888888888888No Hit
GAGCAGAGTTCACACCTTATACTTAAACTTATCGTT11.3888888888888888No Hit
GTGTACCACTAGACGTTGTCGGGGACGTGGATTTAC11.3888888888888888No Hit
TGATATAACTCATCAGTAAATCCAATGGTGCAATTG11.3888888888888888No Hit
CAATCAATGTTAAAGCCATTCACTTTACAAACCACA11.3888888888888888No Hit
ACATATATGACTACGACTTTGAAACACAACTGGAGA11.3888888888888888No Hit
GCTTTGGTGTGAATCAATGTTTGGCATTTAGTGCAT11.3888888888888888No Hit
TATATTGACAACGAAGATACTGCGAACTCATGGAAT11.3888888888888888No Hit
AATATTAACAAAGACAGGTAATATAAGTAAAGACTA11.3888888888888888No Hit
GTGGATATAGGCGGAATGCGCAACACGTCGACTTTT11.3888888888888888No Hit
CCTTCACAACGGCCAATTTGTTTAATCATTCATTAA11.3888888888888888No Hit
CTATTGTAAGAAAGAGGGACATCGCCGGATCGAGTG11.3888888888888888No Hit
ATATTAAGTCTATTTGAAATTTAGTCTTGTATGCGT11.3888888888888888No Hit
GGTCCGGTGCGATGCAGAATCACATATTCACCCCAC11.3888888888888888No Hit
CAATAGTCCTGACGTAGGTACTAGCAGTGGAAATCG11.3888888888888888No Hit
GTTCATAGCAACAATAACAGGTAATTCTAAGGTGGC11.3888888888888888No Hit
ACTTATATGATATTACGAATCAAACGCTAACGCTTT11.3888888888888888No Hit
ATATAGTGTAATGCGGCTAAATGTTAGTGCAGGAAA11.3888888888888888No Hit
CACACCAACCACATTTCAATCTCTACAGCCACAAAA11.3888888888888888No Hit
CATTTTTACTGTGCGAACCATGAAGCAAGGTGGCAA11.3888888888888888No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATGCT50.029.99999816
CTTTATT50.029.99999824
AAACATA50.029.9999987
GATGCTT50.029.99999817
TAAAAAC50.029.9999984
TTATTGT50.029.99999826
TATAAAA50.029.9999982
TATCGGA50.029.99999812
TCGGATG50.029.99999814
TATTGTG50.029.99999827
CGGATGC50.029.99999815
GTATAAA50.029.9999981
ACATATC50.029.9999989
TTCTTTA50.029.99999822
ATTGTGG50.029.99999828
ATAAAAA50.029.9999983
ATCGGAT50.029.99999813
TGCTTCT50.029.99999819
ATGCTTC50.029.99999818
TGTGGGG50.029.99999830
Produced by FastQC (version 0.11.2)