FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_70.352000000397c0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_70.352000000397c0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences575263
Sequences flagged as poor quality0
Sequence length36
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9780.17000919579392382No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGAAC400.006511533618.7475573
CTAGGGT400.006511533618.7475573
GGTATAA600.003745465315.0032611
TACACCG1251.13115675E-714.3981235
GTATTAG952.6252384E-514.2136151
CACCGAT1004.2684776E-513.498247
GTTATAA902.6434715E-413.3362311
TCTGGAC902.6512655E-413.33159353
GTGTATA800.001651950213.1278531
GGACCGT800.001656282113.1232896
TAGAACA850.002612078612.3513324
ACCGATC850.002612078612.3513328
GTAATAC850.002612078612.3513323
TAATACT1101.0424203E-412.2711264
GTATATA2001.720764E-912.0026081
ATAATCC900.004006827711.6651453
TGTAAGG900.004006827711.6651452
GTATAAT1551.5345522E-611.6154271
TAGGAAT1059.7085023E-411.4270814
ACCTCTG950.00599625511.051197
Produced by FastQC (version 0.11.2)