FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_67.35200000039706.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_67.35200000039706.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences849122
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12880.1516860945776932No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTCAG550.002098417416.3666922
AACTGTG859.212174E-615.87970357
GTGTAAG809.7026095E-515.0081021
GTATATG1851.8189894E-1214.6024771
GAACCCC902.6544134E-413.3311096
GTGTATA1601.2003511E-813.132091
ATTACAC1151.0940259E-513.0413023
TGTAGAC1056.756278E-512.8549983
CTAGAAC954.1913133E-412.6294713
ACAGAAC954.1913133E-412.6294713
GTAACAC1201.6949258E-512.4979143
TTATACT5050.012.47316554
GTACTAG850.002600734112.3596141
ATCCCTC850.002614561212.35088112
ACTGTGA1101.0439039E-412.270688
TCTTATA3150.011.9027762
TAAGGGC900.004010606611.6647214
TTAGTAC1859.160067E-811.3494573
TCTAGGA950.006001869711.0507872
GACTTAG950.006001869711.0507877
Produced by FastQC (version 0.11.2)