FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_65.3520000003969b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_65.3520000003969b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1890931
Sequences flagged as poor quality0
Sequence length36
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22000.1163448058125865No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTAGG1354.2157208E-612.22121052
TACACCG3750.011.5984265
GTGTTAA1303.836312E-511.5414731
GCTCTAG1100.001430305810.9119381
GTATTAG3600.010.8361611
TAACCAG1551.9586674E-510.6437175
TCTTACA2002.6916496E-710.4991292
CTAGGGC1304.923624E-410.3832053
GTCTAGG1602.7684824E-510.3151911
GTCTATA3650.010.2766551
ACAGTGC2105.262373E-79.9986438
GTATATA6550.09.8498981
TCTAGTA2157.2554394E-79.7666322
GTCTAGA2003.0336741E-69.7525451
CATACAC3101.8735591E-109.67610553
TAGGGCT1409.727558E-49.6415484
TGTTAAG1250.0041451799.5992042
TATACCG3152.5283953E-109.5225175
CTCTATA2701.834087E-89.4436632
TAGGCTG1603.1515863E-49.3737285
Produced by FastQC (version 0.11.2)