FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_64.3520000003964b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_64.3520000003964b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1214948
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18670.153669128226064No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGACA2000.014.2491784
GTCCTGT1302.7452934E-612.6978471
CTAGACT850.002613538412.3522294
TAGGGGT1006.452561E-411.9993084
ACAGGTT1501.0403819E-611.9988158
TAGACTG1151.5799921E-411.7379715
TACACCG1303.8477894E-511.5373225
GTGCGAT1059.716173E-411.42791311
TACACGG1059.719523E-411.4274435
TCTACTC1602.2615277E-611.2498153
GGCTAAG1202.3464666E-411.2488897
GACAGGT1755.479651E-711.1417577
TGTGCGA950.006001349511.05153910
ACAGCGT1501.3608964E-510.9989138
GTATTAG2607.6397555E-1110.9663231
TCTAGAC1408.175416E-510.7141093
ACTGTGC1408.182251E-510.7132278
CTATGCA2402.6539055E-910.6239519
GGCTATA1704.651616E-610.5928571
TAGACAG1704.6814766E-610.587195
Produced by FastQC (version 0.11.2)