FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_63.35200000039607.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_63.35200000039607.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1853036
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23420.12638718297971546No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCCCG1402.9940566E-813.9280455
CTAGGAC1056.754781E-512.8570043
GTATTAG3350.012.5415771
TAGGACC1702.8305294E-812.3528074
CTTAGAC2204.9112714E-1112.2725953
ACTGAGC1501.3610446E-510.9995848
CTGAGCG1253.4246684E-410.7995929
TTAGAAC4100.010.6096413
GTCCTAA2002.675897E-710.50357151
AGTCCCC1150.00207643310.4343888
CTTACAC1756.6164466E-610.2856043
TTAGCCT2209.036921E-810.2271624
GGTCGGG1653.8971924E-59.99962214
TATACTG5950.09.8315625
TCTATAC4150.09.7589313
TAGACGG1552.2887207E-49.6770535
TATTAGG4350.09.6550692
ATACCGT4450.09.4378456
CTGGGCA2251.3475947E-69.332989
TAGAACT4350.09.3102454
Produced by FastQC (version 0.11.2)