FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_62.352000000395c2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_62.352000000395c2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1067811
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17980.16838185783813803No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCCG500.001110013318.00033814
TAGGACT901.5831645E-514.9981734
TAGGTAG1950.014.61360555
GGTAGTC1903.6379788E-1214.208137
CTAATAC3500.013.2840973
TCGTGCA800.001658377513.1227879
GAGAGTC1302.7630776E-612.6901687
TTAGGTA2850.012.6300424
TAGACTG954.191288E-412.6300415
ATTAGGT3000.011.998543
ACAGTGC1501.0408658E-611.9979778
ACGGTGT1754.2400643E-811.9979778
TCTATAC2401.8189894E-1111.8735543
CCGGCCC900.00400620511.66688513
CTATGCA2850.011.5769969
GGATCGA1602.2635486E-611.2486316
ACACTGT2155.4096745E-911.1614316
CCAGTAC1355.653363E-511.1097583
CTTAGAC2701.2732926E-1111.1097583
GTATAGG2453.1468517E-1011.0252611
Produced by FastQC (version 0.11.2)