FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_61.3520000003958f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_61.3520000003958f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1492896
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23390.15667534778042141No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACCCC1054.275573E-614.2845034
TAGGGGT952.6382857E-514.2093224
CCGGCCG650.00639081913.84497814
GTATTAG5000.013.5051881
CTAATAC3850.012.8560513
CCTAGAC850.002614555412.3518943
GGGCTCA1303.8486185E-511.53748211
CAGGGCC1303.8486185E-511.5374829
TAGGTAG2351.546141E-1011.4883865
TATTAGG4500.011.332752
TAATACT6500.011.0759834
ACTCTCC1901.326207E-711.0516958
GCAGTGC950.00600181511.0516958
ACAGTCC1653.2760054E-610.9081658
ACCTCGC1100.001434132910.9081658
TAAGGGG1653.2760054E-610.9081655
GCCTCAC1253.422136E-410.80016920
TAAGCAG4200.010.7133775
ACTGTCC1451.16794705E-410.3439498
TTAGGGT1809.257979E-69.9991514
Produced by FastQC (version 0.11.2)