FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_60.3520000003954c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_60.3520000003954c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences675388
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12590.1864113665034025No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTTG750.001016802514.0006919
TTATACT2650.013.0195114
TCTTATA1804.656613E-912.4996922
GTATTAG1856.9740054E-912.1645641
TACACTG1303.8360828E-511.5390325
ATACTAT3350.011.1945826
TAATACT3250.011.077474
GTTCTAG950.005983468611.0548131
CTATGCC950.00599006811.0531779
GTGTATA1501.3539633E-511.0021721
CTATGCA1807.8466655E-710.8338689
ACTATGC2002.6756243E-710.5005188
CCTGTGC1000.00876758310.5005188
TTCTAGG1150.002073269610.43452552
GTTATAC1304.9098127E-410.3843593
TCCTATA1451.1650732E-410.3445732
CTATGAC1602.7699847E-510.3122463
ATAAAGG2208.9967216E-810.227023
TACTATG2352.152592E-810.213277
TACTCTA1356.9440826E-410.0004947
Produced by FastQC (version 0.11.2)