FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_59.35200000039508.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_59.35200000039508.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1151044
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13550.11771921837914101No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGCG601.8669407E-417.4984539
GGCCTAC809.727037E-515.0045421
TACACAG1453.0413503E-914.481485
GCGCCGA750.001017958413.9999826
ACAGTGC1301.8308128E-713.844938
CTAATAC2800.013.3916743
TAGGACA1251.7781913E-613.1988334
TACACCG954.1905468E-412.6304655
CCTAATA2800.012.3208752
GTATTAG3550.012.2572311
TCTAGAC1754.2366082E-811.99893953
GTATATA6500.011.7727941
GACTGCT1151.5797009E-411.7380947
ACAGGTC1202.3460363E-411.2490068
ACACCGT1755.4779593E-711.1418726
GGACTGC1355.652323E-511.110136
CTAGTAC1901.3251156E-711.0516563
TAGGCTG950.00600055611.0516565
TCTATAC1501.3605662E-510.9990283
CTATGCA2053.2045136E-810.974649
Produced by FastQC (version 0.11.2)