FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_58.352000000394c3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_58.352000000394c3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences732132
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12480.17046106439822326No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGGC400.00651154418.7483779
ACTGCCT600.00375434714.99870213
CTCGGTC600.00375434714.9987029
GATTAGG750.001013793414.0064421
TCTAGGG1004.2670912E-513.4997553
CTATCCC800.001655635313.1247614
TAATACT3150.012.8569094
ACTGTCC954.1872484E-412.6304868
AGACTGT850.002612394312.3518726
GTACCCA850.002612394312.3518726
GTCTATA1754.1951353E-812.0055221
ACACTAC1006.445028E-411.9997823
TAAGACT1006.445028E-411.9997824
CTGAACG1151.5781696E-411.7381159
CTAAGCA900.004005254211.6664544
TGGAGTG1059.70491E-411.4283645
GGTTATA1602.2446056E-611.2551761
CTTTAGG1202.342293E-411.2497952
GTAAACC950.005990849311.05318524
AACACTG950.005993895711.052435
Produced by FastQC (version 0.11.2)