FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_57.35200000039480.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_57.35200000039480.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences520410
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTAGG350.002999728521.4337881
AGTGGCC500.001109447317.9991938
ATTAGGT1152.21371E-916.9557613
TTAGGTA1001.4190482E-716.499264
TACACCG550.002100193216.3629045
TAGGTAG859.187832E-615.88163955
GGATAGG706.0178636E-415.0036531
GTATAGC901.5746187E-515.0036531
CACCTAG600.003748967515.00076928
CCAACAC706.031046E-414.9993283
GTATTAG1802.1827873E-1114.1701161
CGATCAC750.001016237214.00071824
TAGGCAG750.001016975713.999372511
CTAGGCA750.001016975713.999372510
GACTAGG750.001016975713.99937258
GAAGTGA650.006381755713.8455326
TAGGTAA1004.2635154E-513.4993955
GAAACGG902.6464317E-413.33401630
TCTGACG800.001654847113.12441219
GTATAAT1907.6397555E-1012.6346541
Produced by FastQC (version 0.11.2)