FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_56.3520000003944d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_56.3520000003944d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences781511
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13940.17837240934548587No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAATA1658.185452E-1114.5454232
GAGAGTC952.6346495E-514.2095867
AGTGTCC952.6346495E-514.2095868
CTAGGAC650.006383728713.8461253
TACAGCG902.6517286E-413.3324527
TCCTATA1251.7743841E-613.1999722
GCCCTAA800.001648442113.1325341
TTAGGTA1804.660251E-912.4999734
ACAGTGC1201.692765E-512.4991748
GCTTTAG1456.8384907E-712.4209191
GTATTAG2204.7293724E-1112.2797731
TATACCG1406.3112893E-611.7849355
TAGGTAG1151.5781319E-411.7383545
AGAGAGT2052.5556801E-911.7065436
TGTGCAG900.00400710211.66589510
AGAGACC1303.8424147E-511.5376988
ATTGACC1059.704886E-411.4285473
GTCTAAA2002.2007953E-811.2564591
TAAGGCA1202.3423291E-411.2499754
TAGGACA1202.3423291E-411.2499754
Produced by FastQC (version 0.11.2)