FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_55.35200000039409.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_55.35200000039409.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1062750
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16290.1532815808045166No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCCG802.4946894E-718.7510925
TCGCCCG400.00650976918.75020624
TCGCTCC951.5070909E-615.79113428
CTAGACA1503.0559022E-1014.9987544
ACAGGCG600.003755748514.9987548
CCCGGAT952.6344936E-514.21068324
ACCGTGC952.6368321E-514.2093468
TAGACAG1802.1827873E-1114.1654885
TCTAGAC1201.1230841E-613.74885753
GTATTAG3300.013.642291
GCGCCGA1106.904078E-613.63715526
CTGGCGC1106.907436E-613.63651323
TAGGTAG2009.094947E-1213.4988785
CCTAGCG902.6492932E-413.33473430
GTCCTAG800.001650853913.1307051
CGCCGAA1151.0921089E-513.04423527
GGTAGTC2102.1827873E-1112.8560747
TATTAGG2750.012.5444122
TTAGGAC1201.6944154E-512.4989613
CTAGGCA1101.04356564E-412.27170710
Produced by FastQC (version 0.11.2)