FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_54.352000000393d1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_54.352000000393d1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1366191
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19450.14236662370049283No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGCT706.041135E-414.9989399
GTATTAG3200.013.1322421
GGGCGTG800.00165756913.1240717
GGTAGTC2307.2759576E-1212.3904287
TAGTCAG1702.8303475E-812.3520679
GGGGTGT1006.4541795E-411.9991516
GTATTAA3450.011.3105841
CTAGACA1602.2636814E-611.2492044
TAGACAC950.006000746511.0518495
TTAGGGT1253.42447E-410.7992364
TAGACTG1551.9561647E-510.6444095
AGCACTG2002.688812E-710.4992575
GTATATA9450.010.4819711
TAGGTAG2053.775076E-710.2431775
TATACAG4700.010.2120435
GTCTAGA1356.9217506E-410.0055171
CTACACA1653.896565E-59.9992934
GGGCTTC1200.00295620099.9992926
ATACAGG2701.724402E-99.9992916
TATTAGG3051.364242E-109.8357292
Produced by FastQC (version 0.11.2)