FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_53.3520000003939e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_53.3520000003939e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences501898
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATATATATACATGAGAAGAACGGCATAGTGCG8280.16497375960852603No Hit
TATATATACAGGCAACACGCAGATATAGGTGCGACG7490.14923350959756765No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7380.14704182921629494No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGAC1251.8189894E-1219.20714629
AGACTGA400.00651399318.7457666
CTAATAC1650.016.3599413
GCGCAGC653.437702E-416.1501987
TGCGACG1501.8189894E-1116.00595530
CAGCTCG755.621592E-515.99638710
GGTGCGA1552.910383E-1115.4880928
CGGAAGC809.712155E-515.00408728
TGCGCAG706.0388027E-414.9966136
GAGAGTC600.003755636314.9966127
AGCTCGC750.001018279113.99683811
GTGCGCA750.001018279113.9968385
ACTGTGC650.006388992513.8430278
ACTGTGT800.001656960613.1220368
TGCAGTC800.001656960613.1220367
GTATAAA2001.2551027E-1012.7534741
CGGCATA2307.2759576E-1212.3946825
GAAGCGC850.002601451512.35753930
CGTTGCA850.002607294412.35384517
GGATAGT850.002613148412.3501516
Produced by FastQC (version 0.11.2)