FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_52.3520000003935b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_52.3520000003935b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences955933
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15190.15890234985087867No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTGC400.00651331918.7482018
ACTGATC1054.272186E-614.2843458
ACCGTGT650.0063890713.8448268
TATACGG2350.013.4022685
CCTATAC2401.8189894E-1212.4981473
TAGGTAG1101.0439879E-412.2709075
GTATAGC1501.0376061E-612.0007321
GGTTATA1551.5409678E-611.6136121
TACTCTG1303.8477316E-511.5367525
GAGTGTG1059.715974E-411.4274767
ATACGGT2103.7380232E-911.4274766
ACAGGCA1859.156429E-811.3502638
TATTAGG2659.094947E-1211.3190772
GTCCTAA1202.3419964E-411.2506871
CCCTATA1202.3465484E-411.2483322
GGTGCGA950.00598796411.05446228
CTCCTGA950.00599262911.0533071
TGACAGC950.00599963311.0515726
ACGGTGT1807.874369E-710.8322948
TATACCG1951.8966784E-710.7676345
Produced by FastQC (version 0.11.2)