FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_51.3520000003930a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_51.3520000003930a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1584103
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22430.14159432814659148No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT550.002103993416.361456
CCTAGGA902.6559387E-413.3315522
GGTGCGA1201.6904947E-512.50267128
CTAATAC4100.012.4373633
CACGCAG1406.3208663E-611.78525516
ACACGGT900.004012231211.6651086
CTAGTAC2206.2937033E-1011.589363
CCTAGCG1059.6995354E-411.43065230
ACTATGC4250.011.2926088
GTGCGAC1355.6380748E-511.11348529
GCGCTGG950.00600427111.05115411
GAGAGTC2104.575668E-810.7128537
ACAGTCC1704.6863443E-610.5868228
ACAGGTC1704.6863443E-610.5868228
GTATATG7850.010.5147881
ACAGGCC1000.00878824110.4985978
CTAGGAG1150.002077802310.4333893
TGCGACG1451.1648981E-410.34671130
TACAGGC2505.2059477E-910.1986377
GTCTAAG2105.214606E-710.004981
Produced by FastQC (version 0.11.2)