FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_50.352000000392c5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_50.352000000392c5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences924549
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15220.16462080430566686No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGCGT805.164684E-616.8771325
CGCCGCG809.7473676E-515.00027124
CAGCTCC851.6341504E-414.1163759
GGCACTG650.00638861613.8449065
CTTACAC1004.2723557E-513.4987833
GGTAGTC1302.7595142E-612.6911637
GTATTAG2051.9099389E-1012.443961
TAGGTAG1456.898281E-712.4126755
GAGTAGA1252.561925E-512.0047621
GTACTAG1252.561925E-512.0047621
CCCTATA1006.4488203E-411.9995672
GTCCTAA900.00399421311.6712961
TGGCCTC900.0040087711.6656155
GTCTAGA1202.334554E-411.2544641
GTGTACA1202.334554E-411.2544641
GTATAGG1602.2484128E-611.2544641
GTATTAA2155.3605618E-911.167221
TATTAGG2453.1832315E-1011.0200112
GCCTAAT1100.00142748810.913421
TCGCCGC1100.00143265410.90869823
Produced by FastQC (version 0.11.2)