FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_49.35200000039282.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_49.35200000039282.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1885905
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25780.13669829604354408No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGCA854.7263347E-717.6455159
CTGTGCG1351.85737E-814.443189
GTATTAG3550.014.3733231
TAGGCTG902.6553217E-413.3321685
GGCTCGG1151.0882555E-513.0499491
ACTGTGC1855.0749804E-1012.9718388
CGTCCCC1101.0444355E-412.27165410
TATACCG2800.011.7846835
ACCGCGC1151.5807139E-411.7381038
TCGGGGG900.004008949711.66657416
TAATACT6700.011.6407734
GTCTAGA2351.5279511E-1011.4950621
CTAGGAC1059.7229576E-411.4275713
CTAATAC4800.011.2490163
CTGTGTG2155.4187694E-911.1618149
GTCGGGG950.00599937411.05254415
CCTAATA4800.010.9365432
ATCACAC2802.7284841E-1110.71533729
CTTACAC2251.1024895E-810.6657333
CGCGCAT1150.002077320810.43386910
Produced by FastQC (version 0.11.2)